Fermenter Pilot
fermenter
designed with special features for work under aseptic conditions. It is equipped with magnetically coupled agitator, flat turbine impeller, that easily can be changed e.g. to marine type propeller. The aeration system allows air flows of up to 2 VVM. Electrode and sample ports are placed in side and in the top cover. Sight glass in top cover. The sparger is easily exchangeable or removable for different methods of aeration. The vessel can be provided with central draft tube. To facilitate cleaning and preparation, the fermenter is mounted tiltable on a movable stand.
Piping and Control Unit The fermenter is provided with
piping and control system enclosed in a stainless steel, floor standing cabinet or of open frame type. In the
closed cabinet type the lower part of the cabinet contains valves, circulation pump, heater etc. to conduct gas, steam and water for sparging, sterilisation and temperature control
during the process. The open frame type of system contains the same type of components, but arranged to be easily accessible and visible, fixed on a floor standing skid. The gas
flow can be set with a manually operated valve and is monitored by a mechanical flow meter. The system may optionally include an electronic flow measurement of the gas flow and
regulation through the Novaferm computer control system by sensor and control valve in the gas inlet line. Sterilisation, cooling and fermentation temperature are controlled by
solenoid and pneumatically operated valves, circulation pump and heater governed from the computer control system. Manometers at the front indicate the pressure of the connected
media. The electric cabinet is placed at the upper part and contains the electric components, the microprocessor control system and additional instrumentation. The cabinet is, when
required, provided with fan for forced ventilation. All connections of supply media are placed at the rear of the bottom frame. Control Unit The system is provided
with a microprocessor control system for direct digital control of fermentation processes based on programmable logical controllers from the SIMATIC S7 (Siemens) family. The modular
concept allows control of fermenter systems from laboratory to industrial scale. The standard control system includes hard- and software for control of temperature, speed and pH
and measurement of dissolved oxygen. As an option dissolved oxygen can be cascade controlled by agitator speed and air flow. Additional parameters can be supplied on request. PC
hardware is not included in the standard package. Sensor Kit The standard kit contains the following:
- 2 pcs Pt100 sensor with cable and connectors
- 1 pce Inductive sensor with cable and connector
- 1 pce pH sensor (Mettler) with cable and connector
- 1 pce pO2 sensor (Mettler) with cable and connector
- 1 pce Foam sensor with cable and connector
Additional sensors on request. Pump Unit Peristaltic pump for dosing of acid, alkali or other addition. Fix speed pump. Including glass container, tubing, sterile
filter and needles. Container, hose and needles to be sterilised in autoclave. Delivered with silicone hose diameter 6,4/3,2 mm. Technical Specification Fermenter Vessel, 140 L Vessel
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum
Conditioning, vessel interior: Ground, grit 220, electropolished
Conditioning, vessel exterior: Ground, grit 220, electropolished
Bottom: Dished, with agitator
Jacket
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum Lid
Material: Acid resistant stainless steel equal to AISI 316L
Conditioning: Ground, grit 220, electropolished
Dished, diam. 450 mm, with six clamps Stand
Material: Stainless steel
Conditioning: Ground, grit 180, brushed
On wheels, with tilting possibility Agitator
Bottom mounted, magnetically coupled. Torque 13 Nm, power 1
kW, speed 0-1500 rpm. Impeller, flat turbine, 6 blades. Other powers on request. Tungsten carbide bearings Seals
Material, packings and seals: EPDM Connections
Connections
in lid: 7 pcs E-connections, diam. 25 mm. Sight glass, diam. 80 mm
Connections in side, upper level: Gas inlet, gas outlet
Connections in side, lower level: 5 pcs E-connections, diam. 25 mm
Connections in bottom: 1 pce temp. sensor pocket, diam. 6 mm
Bottom valve for aseptic drain
Connections in jacket: TC-clamp flanges Sparger
Material: Acid resistant stainless steel equal to AISI 316L
Single nozzle below impeller. Multi nozzle, ring type etc. on request Baffles
Material: Acid resistant stainless steel equal to AISI 316L
With 4 baffle plates. Detachable
Pt 100 pocket
Material: Acid resistant stainless steel AISI 316L for Pt 100 sensor Sterile filter
Heated filter housing. Capacity 200 l/min, incl. filter cartridge glass
fibre deep filter type, or 0.22 µm hydrophobic PTFE filter cartridge Accessories
Sampler in acid resistant stainless steel equal to AISI 316L for aseptic sampling and
inoculation. In situ sterilisable
Rubber septum connector with 7 holesPTFE-hoses, stainless steel wire reinforced for connection of jacket to steam/condensate and water/drain,
aeration and filter jacket to steam and drain
Sight glass illumination
Seal kit
Tool kit Volume
Total volume 140 l, working volume approx. 100 l Dimensions
H=1650 mm, W=585 mm, L=850 mm
Vessel diam.=450 mm
Weight, approx. 180 kg Design
According to the Swedish Code for pressure vessels Technical Specification Piping and Control Cabinet for Fermenters 140 L MATERIAL
Cabinet: Stainless steel
Tubing: Stainless steel
Components: Stainless steel, brass/gunmetal DESIGN
As per Swedish regulations for electrical installations. Electrical parts splash protected, mainly IP54 CONNECTION DATA AUX. AIR
Conn. No. 15
Max. consumption 5 l/min free air PROCESS GAS IN
Conn. No. 20
Max. consumption 200 l/min free gas WATER
Conn. No. 20
Max. consumption 50 l/min STEAM
Conn. No. 20
Rec. min capacity 150 kg/h DRAIN
Conn. No. 25
Max. capacity 60 l/min ELECTRICITY
Voltage (see below)
Max. consumption 4 kW Gas filter, in
Conn. No 15 Gas filter, out
Conn. No. 15 Vessel jacket, up
Conn. No. 15 Vessel jacket, down
Conn. No. 15 All
connections for inlet supplies media are provided with female pipe thread type BSP. MEDIA REQUIREMENTS AUXILIARY AIR
Pressure 4-6 bar
Dry, particle free
PROCESS GAS
Pressure 2 bar
Dry, oil-free, particle free WATER
Pressure 2-4 bar
Particle free, max. temp. 5o below min. vessel control temp STEAM
Pressure 2 bar
Dry, saturated, particle free DRAIN
Free flow ELECTRICITY
Voltage 3x380V+N+G, 50 Hz
Transient free, voltage stability +-5% DIMENSIONS
Height=2000 mm, Width=630 mm, Depth=820 mm
Weight, approx. 200 kg |
