Fermenter The
fermenter
is a laboratory/pilot fermenter designed with special features for work under aseptic conditions. It is equipped with magnetically coupled agitator, flat turbine impeller that easily can be changed e.g. to marine type propeller. The aeration system allows air flows of up to 2 VVM. Electrode and sample ports are placed in the top cover, above the liquid level as well as the viewing glass. The sparger for aeration is easily exchangeable or removable for different methods of aeration. The vessel can be provided with central draft tube. To facilitate cleaning and preparation, the fermenter is mounted tiltable on a movable stand.
Piping and Control Cabinet The cabinet
is a floor standing unit built on a frame of stainless steel profiles. It can be provided with wheels for easy moving of the complete system. It contains all necessary controls for the culture vessel.
The lower part of the unit contains valves, circulation pump, heater etc. to conduct gas, steam and water for sparging, sterilisation and temperature control during the process. The gas flow can be set with a manually operated valve and is monitored by a mechanical flow meter at the front of the unit. The system may optionally include also an
electronic measurement of the gas flow, and regulation through the control system by mass flow sensor and control valve in the gas inlet line. The basic gas flow system is for air.
Additional gas flow systems can be added, e.g. for O2 and/or N2. Sterilisation, cooling and temperature are controlled by solenoid and pneumatically operated
valves, the circulation pump and the heater governed from the control system. Manometers at the front indicate the pressure of the connected media. Connections to the fermenter are
placed at the left hand side, with clamp quick-connections for the flexible hoses. The upper part of the unit contains the microprocessor control system and additional
instrumentation. It is placed at the level about 1.5 m from the floor for convenient operation. All connections of supply media are placed at the rear of the bottom frame. The
Electric Unit is mounted in the lower, right part of the unit and contains electric equipment to execute the control signals from the microprocessor control system. The unit comprises
contactors and relays for the circulation pump and heater, fuses, transformers etc. and the agitator speed control unit. Control Unit The system can be provided with the
Novaferm Fermenter Control System for direct digital control of fermentation processes based on programmable logical controllers from the SIMATIC S7 (Siemens) family. The modular
concept allows control of fermenter systems from laboratory to industrial scale. The standard control system includes hard- and software for control of temperature, speed and pH
and measurement of dissolved oxygen. As an option dissolved oxygen can be cascade controlled by agitator speed and air flow. Additional parameters can be supplied on request. PC
software for operator interface, but not hardware is included in the standard package. Sensor Kit The standard kit contains the following:
- 2 pcs Pt100 sensor with cable and connectors
- 1 pce Inductive sensor with cable and connector
- 1 pce pH sensor (Mettler) with cable and connector
- 1 pce pO2 sensor (Mettler) with cable and connector
- 1 pce Foam sensor with cable and connector
Additional sensors on request. Pump Unit Peristaltic pump for dosing of acid, alkali or other addition. Fix speed pump. Including glass container, tubing, sterile
filter and needles. Container, hose and needles to be sterilised in autoclave. Delivered with silicone hose diameter 6,4/3,2 mm. Technical Specification Fermenter Vessel, 75 L Vessel
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum
Conditioning, vessel interior: Ground, grit 220, electropolished
Conditioning, vessel exterior: Ground, grit 220, electropolished
Bottom: Dished, with agitator Jacket
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum Lid
Material: Acid resistant stainless steel equal to AISI 316L
Conditioning: Ground, grit 220, electropolished
Flat, with six clamps Stand
Material: Stainless steel
Conditioning: Ground, grit 180, brushed
On wheels, with tilting possibility Agitator
Bottom mounted, magnetically coupled. Torque 13 Nm, power 1 kW, speed 0-1500 rpm
Impeller, flat turbine, 6 blades Seals
Material, packings and seals: EPDM Connections
Connections in lid: 9 pcs E-connections, diam. 25 mm. Sight glass, diam.
80 mm
Connections in side, upper level: Gas inlet, gas outlet
Connections in side, lower level: 5 pcs E-connections, diam. 25 mm
Connections in bottom: 1 pce temp. sensor pocket, diam. 6 mm
Connections in jacket: TC-clamp flanges Sparger
Material: Acid resistant stainless steel equal to AISI 316L
Nozzle below impeller Baffles
Material: Acid resistant stainless steel equal to AISI 316L
With 4 baffle plates. Detachable Pt 100 pocket
Material: Acid resistant stainless steel equal to AISI 316L Sterile filter
Heated filter housing. Capacity 200 l/min, incl. filter cartridge glass fibre deep filter
type, or, with adapter, 0.22 µm hydrophobic PTFE filter cartridge Accessories
Sampler in acid resistant stainless steel equal to AISI 316L. In situ sterilisable
Rubber septum connector, with 7 holes
Emptying tube for E-connection in lid
PTFE-hoses, stainless steel wire reinforced for connection of jacket to steam/condensate and
water/drain, aeration and filter jacket to steam and drain
Sight glass illumination
Seal kit
Tool kit Volume
Total volume 75 l, working volume 50 l Dimensions
H=1385 mm, W=505 mm, L=700 mm
Vessel diam.=375 mm
Weight, approx. 160 kg Design
According to the Swedish Code for pressure vessels Technical Specification
Piping and Control Cabinet for Fermenters 40-75 L MATERIAL
Cabinet: Stainless steel
Tubing: Acid resistant stainless steel
Components: Mainly stainless steel, some brass/gunmetal DESIGN
As per Swedish regulations for tubing and electrical installations. Electrical parts splash protected, mainly IP54
CONNECTION DATA AUX. AIR
Conn. No. 8
Max. consumption 5 l/min free air PROCESS GAS IN
Conn. No. 15
Max. consumption 100l/min free gas
WATER
Conn. No. 15
Max. consumption 30 l/min STEAM
Conn. No. 15
Rec. min capacity 50 kg/h CLEAN STEAM
Conn. No. 10
Rec. min capacity 10 kg/h (approx. 5 kg/batch culture) DRAIN, WATER
Conn. No. 20
Max. capacity 50 l/min DRAIN, COND.
Conn. No. 10
Max. capacity 1 l/min ELECTRICITY
Voltage (see below)
Max. consumption 4 kW Gas filter, in
Conn. No. 15 (TC) Gas filter, out
Conn. No. 15 (TC) Vessel jacket, up
Conn. No. 15 (TC) Vessel
jacket, down
Conn. No. 15 (TC) Reflux condenser, down
Conn. No. 8 (TC) Reflux condenser, up
Conn. No. 8 (TC) All connections for inlet supplies media are provided
with female pipe thread type BSP. Can optionally be delivered with PTFE-connection hoses. MEDIA REQUIREMENTS AUXILIARY AIR
Pressure 4-6 bar
Dry, particle free
PROCESS GAS
Pressure 2 bar
Dry, oil-free, particle free WATER
Pressure 2-4 bar
Particle free, max. temp. 5o below min. vessel control temp STEAM
Pressure 2 bar
Dry, saturated, particle free CLEAN STEAM
Pressure 1.5 bar
Dry, saturated, pyrogen free DRAIN
Free flow ELECTRICITY
Voltage 3x380V+N+G, 50 Hz
Transient free, voltage stability +-5% |
